Evaluate·Physics linter

§ EVALUATE · Physics linter for any DNA sequence

Evaluate

Paste any DNA sequence to get its thermodynamic profile, CpG islands, structural-form flags, and 13 anti-hallucination flag codes. Single sequences run in-browser; batch via POST /v1/evaluate.

Sequence480 bp · OK

Max 100 kb · Batch via POST /v1/evaluate

§01Summary474 bp · 2 islands

GC content52.4 %balanced 40–60

CpG count4799.2 per kb

Tm est.71.3 °C50 mM Na⁺

⟨ΔG₃₇⟩-8.42kcal/mol · per stack

Flags135 warn · 8 info

§02Thermodynamic profile30 bp window

§03CpG islands detected2 islands

Region	Length	GC	O/E CpG	CpG #
120–250	131 bp	64.2 %	0.78	12
320–410	91 bp	71.0 %	0.84	14

§04Flags5 warn · 8 info

Warn120–250CPG_ISLANDCpG island detected (GC 64.2 %)

Warn320–410CPG_ISLANDCpG island detected (GC 71.0 %)

Warn410–450Z_FORM_HIGHZ-form propensity 0.74 above threshold

Warn240–270TANDEM_REPEAT(TA)ₙ repeat — possible secondary structure

Warn300–320POLY_Apoly-A stretch ≥ 10 bp · pause-prone

Info60–230HIGH_GCRegion GC > 70 % over 170 bp

Info280–310LOW_GCRegion GC < 25 % over 30 bp

Info300–330AT_RICHAT-rich segment (TM est. 58 °C)

Info90–160GROOVE_NARROWMinor groove width compressed

Info300–315A_TRACTA-tract — intrinsic curvature

Info305–340STACKING_LOWStacking ΔG above −7.4 kcal/mol

Info0–450CODON_USAGENo ORF context — codon flags suppressed

Info0–474BATCH_HINTBatch endpoint available for ≥ 100 sequences

Z-form penalty3.8 kcal/mol

Major groove width11.4 Å

Minor groove width5.9 Å